Inventors: Assist. Prof. Dr. Hitaf Abdul Malik Ahmed al-Salim/ Department of Biotechnologies, College of Science, University of Baghdad
Miss Shahad Emas Neima/ Dora, Baghdad
Abstract
Pseudomonas aeruginosa were isolated from different environments including soil and clinical specimens, burns and wounds. The isolates were screened for pyocyanin production using King A medium. B4 isolate (from burn) was the higher pyocyanin producers, reached to 7.172µg/ml. Pyocyanin was extracted using production enhancing media, glycerol alanine minimal medium. The pigment was partially purified by silica gel technique and then dried. Different concentrations of pyocyanin (50, 100, 150µg/ml) were tested for their effect against some plant pathogenic fungi, Rhizoctonia solani, Macrophomina phaseolina and Fusarium solani. The results showed that pyocyanin has antifungal activity against all these fungi, and this effect was depend on concentration.