A New Experimentally Pharmaceutical product for Cutaneous Leishmaniasis Treatment in Iraq Consisting of Gentian violet with Arabic Gum In vivo

A New Experimentally Pharmaceutical product for Cutaneous Leishmaniasis Treatment in Iraq Consisting of Gentian violet with Arabic Gum In vivo

Prepared by:

Amina Neima Thwaini/ Institute of genetic engineering and biotechnologies, University of Baghdad

Abstract

The experiment was conducted on a group of white mice (30) with age of three months and weight between (30-35)g experiment the infection was done  with cutaneous leishmaniasis culture( Leishmania major) by injection subcutaneously , after seven weeks of infection  the skin ulcers were treated with Gentian  violet and Arabic Gum every three days for six weeks , treatment was also used on donors with dermal leishmaniasis 60 Patients who the displaced attending at AL-Yarmouk teaching hospital also every three days for six weeks .The results of the experiments showed healing of the ulcer completely compared to the original treatment of pentostam where the cure is less and the longest period of about a year : The two treatment were also compared (in vitro) on the  medium of the Leishmania containing the infected stage (promastigots) . A tissue study of the animals skin, the  infection  was done by Leishmania parasites obtained from culture media subcutaneously  to identify the degree of variability before and after treatment.