The research was carried out in the vegetables field and plastic houses, During  spring  season 2013 and fall season 2013-2014 to study the effect of spraying CO2 and amino acids solutions on proline content and total yield of cherry tomato under field and protected cultivation. Hybrid Jaguar,F1 used during spring  season and  fall season and the hybrid Summerbrix  used during fall season only. The treatments consist of spraying different levels of CO2 (0,3,6 g.l-1) as Agrosol and different levels of the amino acid as Enraizal at (0,2,4 ml.l-1) on cherry tomato plants. The two experiments were factorial experiments in RCBD with three replicates. Results could be summarized as follows: In first experiment, the interaction between AG2 and EN0 increased the percentage of fruit set, number of  fruit, plant yield, total yield, proline content in the anthers at the end of the season at the rates of 16.46%, 24.96%, 31.54%, 31.71%, 68.84% respectively. AG1EN1 treatment increased   proline content in the leaves by 54.36% at the end of the growing season. In second experiment, the treatment AG2EN2  influenced both Jaguar,F1and Summerbrix hybrids, number of  fruit per plant was increased by 72.95% and 80.24% and plant yield by 61.15% and 60%. The total yield was increased by 60.81% and 59.80% for both hybrids sequentially. Highest percentage of pollen viability at the beginning of the season was 10.15% in AG2EN2 treatment in hybrid Summerbrix, while highest percentage of pollen viability at the end of the season was 153.86% and 22.02% in AG2EN2 and AG2EN0 treatments for the two hybrids respectively. Treatment AG1EN0 increased fruit set percentage by 31.26% and 61.78% for both hybrids respectively. At the end of the season treatment AG1EN1 and treatment AG2EN2 gave significant increases in proline content in leaves of 237.14% and 70.90% for the two hybrids respectively.  The proline content in the anthers was increased by 27.53% and 81.84% in the beginning of the season in treatments AG2EN0 and AG1EN0 while the proline content in the anther was increased by 154.62% and 86.57% at the end of the growing season in the treatments AG2EN2 and AG1EN0 for both hybrids sequentially.