Inventors: Lecturer Dr. Ibtisam Fadil Musa, Assist. Prof. Dr. Lamis Thamer al-Hadidi, Assist. Prof. Dr. Azhar Jawad Al-Musawi, Dr. Shaimaa Rifaat al-Ani/College of Agricultural Engineering Sciences, University of Baghdad
Abstract
The first point of protection Is the selection of bacteria Lactococcus lactis subsp.lactis for being the most prolific bacteria of external sugar Exopolysaccharide, Has been studied optimal conditions for the production of Exopolysaccharide by limiting the components of the food medium, temperature, pH, size of pollen and fermentation time to isolate the bacteria selected and used whey as a natural food medium for the production of multiple EPS sugar from Lactococcus lactis subsp.lactis bacteria.
The process of extracting and refining the multiple sugar produced from the Lactococcus lactis subsp.lactis bacteria and then using two techniques TLC, HPLC, and specific tests. Exopolysaccharide is a multi-type heterozygous polysaccharide made up of monoclonal sugars: Clactose, Ramenose and N-acetyl glucose amine Multi-sugar has been applied as a fermented substance in the cheese industry Substitute for emulsifying salts (potassium sorbate and sodium).