The chemical isomerization has been used to isomerize whey lactose to lactulose by column reactors. The basic sodium aluminate was used in this process. The aluminium is more effective than calcium ion in isomerization reactions, throughout, its intermediate complexes which are minimizing the formation of by-products. The sodium aluminate was used as catalyst in column reactors with 1, 2 and 3 molar concentrations. The sodium aluminate was immobilized on convenient support such as silica gel and sand. The column chemical isomerization by the sodium aluminate was proceeding under various temperature degrees (30, 50, 70 and 90oC) and flow rates of 1, 2 and 3 ml/min. The best temperature degree and flow rate of highly isomerization have been selected. The presence of degradation by-products has been examined. The effective supporting material has been chosen.