Prof. Dr. Muhammad Ibrahim Nader, a faculty member at the Institute of Genetic Engineering and Biotechnologies for Postgraduate Studies, in conjunction with the researcher Ayman Salam Ismail, has obtained a patent from the Central Organization for Standardization and Quality Control (COSQC) entitled: (Detection of genetic polymorphism of Glutathione S-transferase gene in idiopathic male infertility).
The study aimed to investigate the relationship between some forms of single nucleotide polymorphism of GST genes (M1, T1 and P1) with idiopathic male infertility in some Iraqi patients. Blood samples were obtained from Kamal Al-Samarrai Fertility Hospital in Baghdad, where DNA extraction was carried out in two ways, the first was manually using a Wiz-bio- Korea kit, while the second was automatically using a MagPurix- Taiwan kit. The presence of DNA was confirmed by Electrophoresis and the purity of the DNA was measured using a Nano-Drop device (1.8-2.0). Then the researchers used the High Resolution Melt (HRM) Analysis in Real-time PCR to detect the encoding Glutathione S-transferase (SNP rs-1695 (A> G) GST-P1, SNP rs-2266637 (A> G) GST-T1 and SNP rs-4147565 (G>A) GST-M1) that is containing mutations causing male infertility.
