Prof. Dr. Abdul Hussein Moit al-Faisal and Assist. Prof. Dr. Bassima Qassim Hassan who are lecturers at the Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, have recently published a scientific research paper, extracted from a doctoral thesis submitted by the student “Mohammed Fadel Kazim”, entitled “Tandem mass tag-based quantitative proteomic analysis of cervical cancer” published in the Journal of Proteomics classified in Q1 Scopus journals.
The study aimed to study the cervical cancer that is a common cancer disease suffered by women and caused by high-risk human papillomavirus (Hr-HPV), where many potential biomarkers have been proposed for precancerous lesions and cancer diagnosis to shed light on some of these markers studied earlier. This study determined potential biomarkers for cervical cancer diagnosis in regard to HPV genotype by using isobaric labeling quantitative proteomics in samples distributed over five groups, namely histological disorders (CINI, CINII, CINIII). The qPCR (quantitative polymerase chain reaction) technique was used to identify the genotype of papillomaviruses, also KPNA2, MCM2, COL1A1, and DCN were selected based on functional enrichment analysis and validated by Immunohistochemistry (IHC) testing. The results showed that the KPNA2 marker that was compared to other previously reported biomarkers and is a putative biomarker to be validated in further studies, specifically the relationship with HPV load.
Tandem mass tag-based quantitative proteomic analysis of cervical cancer
