Use of new way to feed Iraqi bees to improve the properties of productivity and therapeutic of the honey

Use of new way to feed Iraqi bees to improve the properties of productivity and therapeutic of the honey

Prepared by:

Ikhlas Mohammed Farhan/Ministry of science and techmologies
Dr. Ruqaiba Ali Jijan/

Department of Food Science, College of agriculture, University of Baghdad
Teacher: Lina Qassim Adnan Al-Kinani/

Department of Food Science, College of agriculture, University of Karbala


Mr. Mohammed Abdul Jaleel Mahmood/

Department of plant protection, College of agriculture, University of Baghdad


Abstract 

The aim of this patent is  to use an innovative new way to feed bee colonies, which return to local strain of  Honey bee (Apis mellifera L.) in Iraq  by using supplementary feeding represented by  sucrose dissolved in magnetized water, which has an  important role  as following:

-Improve Performance of life at honey bee and Represented by (  rate of egg  ,  brood  area   , pollen space and   honey space )

-Population growth of honey bee colonies when you follow innovative way compared  traditional way

-effect  of feed sugar dissolved in magnetized water,   on  worker bees activity in  an  early  stage and collect pollen during different time periods of the day 

-Increase the amount of honey product  was  42.2%  compared to the amount of honey producer in the traditional way.

-increasing protective effect of honey formed by feeding bees  by using innovative way, honey (code honey D)  with honey formed by conventional nutrition which always using a general water with sugar ( code Honey B ),   and compare results them  with honey formed by- natural feed sources (code honey A) , as follow: 

-Reduced significantly in chromosomal aberrations (CA) and Micronucleus (MN), and increased significantly in Mitotic index (MI),   increasing  with    the  treatment period   

-Recording significant  decrease  in  MI  and significant  increase  in  MN,  CAs  ,  in      animals groups  treated with  CP (positive control) compared with negative control

-Improved biomarkers, (MI, and CA, and MN ,) when interaction between  honey D and  Cyclophosphamide (CP) .  honey D  achieved the best effectiveness   to reduce genotoxicity effect  of  CP which showed nearly the same values of  negative control ,   the cytogenetic effects was  similar with   comparison group (honey A) and negative control  in all treatment

-Honey B that  formed by conventional feeding using a general water with sugar was less effective than honey D to reduce genotoxic effect of CP compared with the comparison group (honey (A) and negative control  

-The histopathological examination of liver treated with  cyclophosphamid revealed necrosis of hepatocytes surrounding central venule, ,  and cells lining the blood vessels and additionally,  infiltration of inflammatory cells,  and this indicates severe damage of the liver tissues. In contrast, honey D were able to modulate these effects, and  improvement  in  liver  tissue , Liver  restoration  of  normal histological  structure,The results of honey D came similar with the results of  honey  A   in all  treatment  , this study showed  the importance of honey D to provide liver protection by lowering the values of these indicators, or make it part of the normal level and repair of CP damages the liver, while some  histological changes CP-induced was  continued in histological sections of  liver , when  treatment with honey B

-Histopathological study of the liver  was  also  confirmation  for  histopathological study of the kidneys , microscopic examination  of the kidneys in the  positive  control (CP)  cyclophosphamide  demonstrated  many  histopathological  changes,   it  was  observe shrinkage of the  glomeruli  ,  Expansion in Bowman’s capsule cavity and infiltration of inflammatory cells and mononuclear and expansion of the urinary tubule.  all pathological changes  has been  removed  and  improved the kidneys architecture  in animals treated with honey D at  first place, and then honey A,  While treatment with honey B did not show the same efficiency in reducing the pathological changes  compared   with CP group  

-A significant  decrease in the activity  of  ALT, ALP   and  AST  in  the  serum  of  aluminium  in mice group  treated  with  kinds of honey A, D, as  compared with the  negative control, While Honey B achieved less effective in reducing the  levels of these enzymes activities  induced  by  CP    

-Results of honey D was  similar with the results of  comparison group (honey A), in  reducing increase in the  levels of urea, creatinine when compared to CP-treated animals,  which indicates its ability to improve renal function. Meanwhile, Honey B was less efficient in reducing the proportion of creatinine and urea in all  treatment .

-Highest protective action when  honey D  given pre and simultaneous treatment with the drug  CP , and to less  effect  in  post  treatment.  which means that the  honey D  considered as Desmutagens at the first degree and Bioantimutagens at the second degree.