Inventor: Prof. Dr. Assis Prof . Hameed abbood Jebur/College of Agricultural Engineering Sciences, University of Baghdad
Mustafa Mohamed Omar/College of Agriculture,Kirkuk University
Abstract
An isolate of local bacteria which efficiently distinguished in alkaline protease production was obtained. This enzyme was used in some of application fields which requires to be active in the alkaline conditions. This isolate was identified a Bacillus cereus MH12 based on morphological, cultural characteristics, Vitek2 system and genetic analysis. To increase alkaline protease productivity from this locally isolate, the optimum condition for enzyme production has been studied by submerged state fermentation, then the enzyme was partially purified by precipitation with 30-70 % of saturation of ammonium sulfate followed by dialysis and ion-exchange chromatography, finally the enzyme extract was subjected to gel filtration.
The characteristics of the purified enzyme was studied in order to identify it’s suitability when it use in some industrial applications. These characteristics were included optimum pH and temperature for activity and stability, enzyme specificity toward some of substrates and effect of some ionic metals on enzyme activity. The efficiency of Alkaline protease produced from local isolate Bacillus cereus MH12 were studied in removing of proteins spots from cotton cloth and compatibility of enzyme used with some of commercial detergent powder in local markets . The enzyme also used with high efficiency as alternative to chemicals such as sodium sulfide 2% and calcium oxide 10% to remove hair from cow skin when treat skin with 100 % enzyme ( 612.2 U/ ml ) for 24 h at 50 0 C.