{"id":2864,"date":"2018-04-16T10:15:17","date_gmt":"2018-04-16T07:15:17","guid":{"rendered":""},"modified":"2019-01-28T09:15:16","modified_gmt":"2019-01-28T06:15:16","slug":"decolorization-of-textile-dyes-by-animmobilized-laccase-produced-from-local-isolate-of-pseudomonas-aeruginosa-sr3-using-packed-bed-reactor","status":"publish","type":"post","link":"https:\/\/en.uobaghdad.edu.iq\/?p=2864","title":{"rendered":"Decolorization of Textile Dyes by anImmobilized Laccase Produced from Local Isolate of Pseudomonas aeruginosa SR3 Using Packed Bed-Reactor"},"content":{"rendered":"<div style=\"text-align: justify\"><\/div>\n<div style=\"text-align: justify\"><strong><span style=\"font-size: 14px;font-family: Calibri\">\u00a0<\/span><\/strong><\/div>\n<div style=\"text-align: justify\"><strong><span style=\"font-size: 14px;font-family: Calibri\">Prepared by:<\/span><\/strong><\/div>\n<div style=\"text-align: justify\"><strong><span style=\"font-size: 14px;font-family: Calibri\">Teacher Sahar Irhaim Hussein\/ Department of biotechnologies,\u00a0 Faculty of science, University of Baghdad<\/span><\/strong><\/div>\n<div style=\"text-align: justify\"><strong><span style=\"font-size: 14px;font-family: Calibri\">Dr. Nazim Hassan Haider\/ Department of biotechnologies,\u00a0 Faculty of science, University of Baghdad<\/span><\/strong><\/div>\n<div style=\"text-align: justify\"><strong><span style=\"font-size: 14px;font-family: Calibri\">Dr. Ghazi Muneim Aziz\/ Department of biotechnologies,\u00a0 Faculty of science, University of Baghdad<\/span><\/strong><\/div>\n<div style=\"text-align: justify\"><strong><span style=\"font-size: 14px;font-family: Calibri\">\u00a0<\/span><\/strong><\/div>\n<div style=\"text-align: justify\"><strong><span style=\"font-size: 14px;font-family: Calibri;color: #0070c0\">Abstract<\/span><\/strong><\/div>\n<div style=\"text-align: justify\"><span style=\"font-size: 14px;font-family: Calibri\">\u00a0<\/span><\/div>\n<div style=\"text-align: justify\">\n<p><span style=\"font-size: 14px;font-family: Calibri\">The present study was aimed to production laccase enzyme from the local isolate of Pseudomonas aeruginosa SR3 and using immobilized laccase for removing the different textile dyes by batch and continuous system.Laccase was produced by local isolate of Pseudomonas aeruginosa SR3 using lab scale fermenter after 24 hr of fermentation, the specific activity was reached to 6214 U\/mg proteinwhen xylene concentration was nearly depleted from the medium in the fermenter . Immobilization of enzyme was conducted in two methods, entrapment by calcium alginate and agar-agar with maximum immobilization efficiency 49% and 32%, respectively and covalent method by chitosan and gelatin with maximum immobilization efficiency 63% and 50% respectively. The decolorization capability of immobilized laccase on chitosan loaded with black dye at concentration of (30 mg\/l) in packed bed-reactor with pH 5.0 at 30oC operated as a batch culture system for 3 hrs. Decolorization rate was increased through the incubation time and stabilized approximately after 2 hr with removal efficiency 97.3% of dye. Decolorization of black textile dye was performed also by operation of packed bed bioreactor continuously at different flow rates (15, 30 and 45) ml\/hr and HRT (7.9, 3.9 and 2.6) hr. respectively. The black textile dye was loaded over the chitosan immobilized laccase with total working volume of 118 ml at concentration of (30 mg\/l) in pH 5.0 at 30oC. Decolorization efficiency of black textile dye with immobilized laccase in a bioreactor increased at flow rate 15 ml\/ hr., and reached to 71% after 2.5 hr. While the increase in flow rate to 30 and 45 ml\/ hr. resulted in dropping of decolorization rate of dye to 60 and 47% respectively after period of time.<\/span><\/p>\n<p>&nbsp;<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u00a0 Prepared by: Teacher Sahar Irhaim Hussein\/ Department of biotechnologies,\u00a0 Faculty of science, University of Baghdad Dr. Nazim Hassan Haider\/ &#8230; <\/p>\n","protected":false},"author":5,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_lmt_disableupdate":"","_lmt_disable":"","footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"class_list":["post-2864","post","type-post","status-publish","format-standard","hentry","category-Patents"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.8 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Decolorization of Textile Dyes by anImmobilized Laccase Produced from Local Isolate of Pseudomonas aeruginosa SR3 Using Packed Bed-Reactor - University of Baghdad<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/en.uobaghdad.edu.iq\/?p=2864\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Decolorization of Textile Dyes by anImmobilized Laccase Produced from Local Isolate of Pseudomonas aeruginosa SR3 Using Packed Bed-Reactor - University of Baghdad\" \/>\n<meta property=\"og:description\" content=\"\u00a0 Prepared by: Teacher Sahar Irhaim Hussein\/ Department of biotechnologies,\u00a0 Faculty of science, University of Baghdad Dr. Nazim Hassan Haider\/ ...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/en.uobaghdad.edu.iq\/?p=2864\" \/>\n<meta property=\"og:site_name\" content=\"University of Baghdad\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/uobaghdad.edu.iq\" \/>\n<meta property=\"article:published_time\" content=\"2018-04-16T07:15:17+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2019-01-28T06:15:16+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/en.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/2\/2021\/09\/University-of-Baghdad.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"1700\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"English admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/en.uobaghdad.edu.iq\/?p=2864#article\",\"isPartOf\":{\"@id\":\"https:\/\/en.uobaghdad.edu.iq\/?p=2864\"},\"author\":{\"name\":\"English admin\",\"@id\":\"https:\/\/en.uobaghdad.edu.iq\/#\/schema\/person\/2cb5e415b1c0c8f9ce37a1e4f0431361\"},\"headline\":\"Decolorization of Textile Dyes by anImmobilized Laccase Produced from Local Isolate of Pseudomonas aeruginosa SR3 Using Packed Bed-Reactor\",\"datePublished\":\"2018-04-16T07:15:17+00:00\",\"dateModified\":\"2019-01-28T06:15:16+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/en.uobaghdad.edu.iq\/?p=2864\"},\"wordCount\":315,\"publisher\":{\"@id\":\"https:\/\/en.uobaghdad.edu.iq\/#organization\"},\"articleSection\":[\"Patents\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/en.uobaghdad.edu.iq\/?p=2864\",\"url\":\"https:\/\/en.uobaghdad.edu.iq\/?p=2864\",\"name\":\"Decolorization of Textile Dyes by anImmobilized Laccase Produced from Local Isolate of Pseudomonas aeruginosa SR3 Using Packed Bed-Reactor - 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