{"id":21398,"date":"2019-12-11T13:00:58","date_gmt":"2019-12-11T10:00:58","guid":{"rendered":"http:\/\/en.uobaghdad.edu.iq\/?p=21398"},"modified":"2020-01-28T13:16:37","modified_gmt":"2020-01-28T10:16:37","slug":"a-promising-treatment-for-cancer-the-edible-mushroom-agaricus-bellaniae-that-is-discovered-in-the-hot-iraqi-environment-for-the-first-time","status":"publish","type":"post","link":"https:\/\/en.uobaghdad.edu.iq\/?p=21398","title":{"rendered":"A promising treatment for cancer: The edible mushroom( Agaricus bellaniae ) that is discovered in the hot Iraqi environment for the first time"},"content":{"rendered":"<p style=\"text-align: justify\"><strong>Inventors: Dr.\u00a0Ruqaiba Ali Jijan,\u00a0Dr.\u00a0Zeina Mohammed Abdul Qadir,\u00a0Ikhlas Mohammed Farhan\/Ministry of Science and technology, Dr.\u00a0Jassim Mohammed Udah\/Department of Food\u00a0Sciences, College of\u00a0Agricultural Engineering Sciences,\u00a0University of Baghdad<\/strong><\/p>\n<p><span style=\"color: #3366ff\"><strong>Abstract<\/strong><\/span><\/p>\n<p style=\"text-align: justify\">This work represented a study of the effect\u00a0 of crude extracts of fungi on adenocarcinoma (Ahmed- Mohammed \u2013 Nahi-2003, AMN-3) cell line, the human vitro and\u00a0 in vivo experiment . For the purpose of achieving this goal. The study included four stages. In stage one: The study has also employed an in vitro evaluation of the cytotoxic effect of fungi\u00a0 extract\u00a0 on some cell lines including the murine mammary laryngeal carcinoma (Hep-2) cell line and the rat embryo fibroblast (Ref) cell line, at different concentrations and different treatment exposure time. In stage two: Evaluation of the effect of these extracts in vitro on\u00a0 on several cytogenetic parameters such as mitotic index (MI%), blast index (BI%), The third stage: evaluate preventive activity of\u00a0 aqueous and alcohol extracts on human lymphocyte in vitro by several cytogenetic parameters such as Sister Chromatid Exchange (SE), Cell Cycle rogression (CCP), Replicative Index (RI) Chromosomal aberration (CA) and micronucleus formation (MN) . In stage four: In vivo biological experiments. In stage\u00a0 five ;This work also included study of the therapeutic potential of two extracts,\u00a0 aqueous and alcohol\u00a0 extracts of fungi in the treatment of transplanted murine mammary adenocarcinoma in mice.<\/p>\n<p style=\"text-align: justify\">The in vitro cell growth assay showed that there was time- and concentration-dependent cytotoxic effects of crude extracts of fungi on Hep2 and AMN3 cell lines without any toxic effect in the normal cells of REF cells. The highest significant effect of these extracts was achieved after 72 hrs of exposure with highest concentration (10000 \u03bcg\/ml). Both aqueous and alcohol extract of\u00a0 fungi\u00a0 caused growth inhibition percentage\u00a0 for Hep2\u00a0 through (24, 48, 72 hr.) exposure time ( 48.5% , 64.5% and 89.4 %, respectively) for aqueous extract of\u00a0 fungi .\u00a0 and (37.2%, 56.7% and 78.2%, respectively) for alcohol extract of\u00a0 fungi. However, 72 hrs exposure to crude extracts of fungi at concentration of 10000 \u03bcg\/ml caused slightly inhibitory effect on REF cell line, reached 21.1% and 17.7% for aqueous\u00a0 and\u00a0 ethanolic extracts of fungi, respectively.<\/p>\n<p style=\"text-align: justify\">On the other hand, all crude extracts of fungi caused significant reduction in the mitotic index and blast index of peripheral human lymphocytes, but without any structural or numerical chromosomal aberration. Also these extracts neither replaced phytohemagglutin in (PHA) as mitogenic agents, nor colcemid as mitotic arresting agents at metaphase. Cytotoxic assay of\u00a0 extracts on human lymphocyte in vitro, The non cytotoxic concentrations of both fungi extracts\u00a0 aqueous and alcoholic\u00a0 were:\u00a0 0.1\u00b5g\/ml and\u00a0\u00a0 0.01\u00b5g\/ml , the resulted improved CCP,increased RI significantly, This Positive effect\u00a0 was decreased of SCE ,\u00a0 while the high\u00a0 concentrations resulted decreased of RI and increased of SCE significantly, in addition negative effect of CCP.<\/p>\n<p style=\"text-align: justify\">\u00a0when test the protective effect\u00a0 of non cytotoxic concentrations, which included two treatments (before and after drug CP)\u00a0\u00a0 both extracts showed highly performance in preventing or reducing the genotoxic effect of CP contributed to a decrease in RI and SCE, addition positive effect in CCP,\u00a0 compared with positive control (CP ) . This was supported by the results of a two-dose of\u00a0 study (250 and 300 mg \/ kg) for both fungal extracts on recording some cytogenetic markers\u00a0 in the bone marrow of the micethrough three types of interactions treatment\u00a0 (before, after, with treatment).\u00a0 the results showed that the both extracts\u00a0 administration confers a protection against damage inflicted by CP by decreased chromosomal aberration and micronucleus formation . The positive effect was overt when\u00a0 fungi extracts were used as (pre-CP) and (with-CP) treatments, and to less extent in (post-CP) treatment, So the\u00a0 fungi extracts can be considered as Desmutagens at the first degree and Bioantimutagens at the second degree.<\/p>\n<p style=\"text-align: justify\">The therapeutic doses of both\u00a0 aqueous and alcoholic\u00a0 were determined according to LD50 in mice. The results indicated high effectiveness of both extracts in a dose- and time-dependent manner. respectively. The comparison of relative tumor volumes of different groups revealed very significant differences among all treated groups and those of untreated (control) group. Treated mice with the high and medium doses of aqueous extraction show high reduction ( 86.6 % and 64.5 % ) while the low doses show partial reduction with 52.1 % growth inhibition. The result indicate that\u00a0 treated mice by high doses (1.5 g\/kg) of methanol extraction (77.8%) . Reduction by the medium dose (0.7 g\/kg %) was 68.612 %. The low dose (0.4 g\/k) reduction with\u00a0 growth was 47.5 %. The highest therapeutic doses of aqueous and alcoholic of fungi (\u00a0 and 2.3 g\/kg \u00a0and 1.5 g\/kg , respectively) showed the best therapeutic effect by decreasing the tumor volume in mice to about\u00a0 99% and 98%.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Inventors: Dr.\u00a0Ruqaiba Ali Jijan,\u00a0Dr.\u00a0Zeina Mohammed Abdul Qadir,\u00a0Ikhlas Mohammed Farhan\/Ministry of Science and technology, Dr.\u00a0Jassim Mohammed Udah\/Department of Food\u00a0Sciences, College of\u00a0Agricultural &#8230; <\/p>\n","protected":false},"author":5,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_lmt_disableupdate":"no","_lmt_disable":"","footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"class_list":["post-21398","post","type-post","status-publish","format-standard","hentry","category-Patents"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v25.8 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>A promising treatment for cancer: The edible mushroom( Agaricus bellaniae ) that is discovered in the hot Iraqi environment for the first time - University of Baghdad<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/en.uobaghdad.edu.iq\/?p=21398\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"A promising treatment for cancer: The edible mushroom( Agaricus bellaniae ) that is discovered in the hot Iraqi environment for the first time - University of Baghdad\" \/>\n<meta property=\"og:description\" content=\"Inventors: Dr.\u00a0Ruqaiba Ali Jijan,\u00a0Dr.\u00a0Zeina Mohammed Abdul Qadir,\u00a0Ikhlas Mohammed Farhan\/Ministry of Science and technology, Dr.\u00a0Jassim Mohammed Udah\/Department of Food\u00a0Sciences, College of\u00a0Agricultural ...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/en.uobaghdad.edu.iq\/?p=21398\" \/>\n<meta property=\"og:site_name\" content=\"University of Baghdad\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/uobaghdad.edu.iq\" \/>\n<meta property=\"article:published_time\" content=\"2019-12-11T10:00:58+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2020-01-28T10:16:37+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/en.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/2\/2021\/09\/University-of-Baghdad.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"1700\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"English admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/en.uobaghdad.edu.iq\/?p=21398#article\",\"isPartOf\":{\"@id\":\"https:\/\/en.uobaghdad.edu.iq\/?p=21398\"},\"author\":{\"name\":\"English admin\",\"@id\":\"https:\/\/en.uobaghdad.edu.iq\/#\/schema\/person\/2cb5e415b1c0c8f9ce37a1e4f0431361\"},\"headline\":\"A promising treatment for cancer: The edible mushroom( Agaricus bellaniae ) that is discovered in the hot Iraqi environment for the first time\",\"datePublished\":\"2019-12-11T10:00:58+00:00\",\"dateModified\":\"2020-01-28T10:16:37+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/en.uobaghdad.edu.iq\/?p=21398\"},\"wordCount\":769,\"publisher\":{\"@id\":\"https:\/\/en.uobaghdad.edu.iq\/#organization\"},\"articleSection\":[\"Patents\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/en.uobaghdad.edu.iq\/?p=21398\",\"url\":\"https:\/\/en.uobaghdad.edu.iq\/?p=21398\",\"name\":\"A promising treatment for cancer: The edible mushroom( Agaricus bellaniae ) that is discovered in the hot Iraqi environment for the first time - 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