Within the cultural season of the department of biology at the faculty of education for pure sciences/Ibn al-Haytham, a lecture entitled (hyperoxia: life or death of lung cells) was delivered at the seminar hall by the instructor D. Hanadi Salem Abdul-Sahib al-shamkhani that dealt with hyperoxia which contributes in preservation of the lives of the patients who are suffering from respiratory failure that is leading to pathological consequences including inflammation resulting from oxidative tissue damage, pulmonary fibrosis, pulmonary edema, cilia weakness and pulmonary cell’ death or chronic vulnerability. The damages caused by hyperoxia are as follows:
-Tight junction protein detection
-Pro-inflammatory cytokines detection
-Measurement of transepithelial resistance (TER)
-Immunofluorescence detection of ZO-1, polyclonal rabbit ZO-2, confocal microscopy, Neuron J tracing too
-Detection of cytokines in the medium by ELISA
-Detection of cytokines expression by RT-qPCR
-Cytokines of interest IL-6, IL-8 and TNF-α
-Detection of ZO-1 expression by RT-PCR.
The study showed the following:
1 . Hyperoxia may not lead the death of pulmonary cells sometimes, but to a damage of cellular links which lead to alienation or separation of these cells.
2 . Antioxidants such as vitamins (C, E) provide partial protection from cilia loss caused by hyperoxia
3 . The low level of protein (ZO-1) in pulmonary cells’ links associated with a high proportion of cytokines that cause inflammation.
4 . Antioxidant vitamins played a protective role in maintaining the integrity of the cells.